IN VITRO ANALYSIS OF CALLUS INDUCTION IN INTERSPECIFICALLY HYBRIDIZED F4-5 POPULATIONS OF BRASSICA

Abstract

Explants of F4-5 populations of 3 B. napus (A-20-80, Maluku and Dunkled) interspecifically hybridized with 3 B. campestris lines (1203, 2163 and 2065) were cultured on MS-medium + 2, 4- D (0.5 mg l-1) + Kinetin (0.25 mg l-1) for In vitro analysis. On average, frequency of callus induction was better in F4-5 populations and B. napus species than B. campestris for seed, cotyledonary and leaflet explants. The B. napus parental lines were better than F4-5 population in inducing callus in explants of leaflets and cotyledons. The callus induction was the earliest in F4-5 population as compared to both B. napus and B. campestris species. Callus induction in seed explants of B. napus was late, while early in leaflet and cotyledonary explants as compared to B. campestris.