EFFECTS OF EXOGENOUS GROWTH REGULATORS ON CELL SUSPENSION CULTURE OF “YIN-HONG” GRAPE (VITIS VINIFERA L.) AND ESTABLISHMENT OF THE OPTIMUM MEDIUM

Abstract

Callus induced by stem of "Yin-hong" grape (Vitis vinifera L.) was used as materials and B5 medium as basic medium. The major growth parameters of cell suspension cultures with various levels of 1-Naphthaleneacetic acid(NAA) and 6- Benzyl aminopurine(6-BA)were investigated to provide a basis for the optimum medium of suspension cell cultures of “Yinhong” grape regarding cell number, packed cell volume (PCV), dry cell weight (DCW), cell viability, and morphology. All data were analysed by of two-way analysis of variance (ANOVA). Results showed that the treatment of 6-BA and NAA would effect the cell growth dynamics, probably causing logarithmic phase in advance at higher levels of 6-BA. Different concentration of 6-BA and NAA had significant effects on cells' number, PCV, DCW and viability (p<0.05), while nosignificant effect was observed on the cells morphology. The optimum medium for suspension cell cultures of “Yin-hong” grape was identified asB5+1.5 mg/L6-BA+1.5 mg/LNAA+ 250 mg/L casein hydrolysate + 30 g/L sucrose. With the optimum medium, the maximum number of suspension cells after the logarithmic growth phase was 34.78 × 108 / mL, the highest cell viability reached 86.45%.; DCW reached 3.84 g/L and PCV reached 0.092 mL/mL after eight days cultivating.