LOW TEMPERATURE SCANNING ELECTRON MICROSCOPIC STUDIES ON THE INTERACTION OF GLOBODERA ROSTOCHIENSIS WOLL. AND TRICHODERMA HARZIANUM RIFAI

Abstract

Low temperature scanning electron microscopic (LTSEM) studies revealed that Trichoderma harzianum infected mature potato cysts nematode eggs by penetrating directly the cyst wall or via natural opening of mouth. Mycelial penetration on cyst wall or egg surface has been seen. The penetration of cyst wall or egg surface was either chemical or mechanical (directly or with appresorium) or both. Freeze fractionation showed the presence of mycelia inside the eggs.