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Title: | EXPRESSION ANALYSIS OF FUSARIUM WILT RESISTANCE GENE IN MELON BY REAL-TIME QUANTITATIVE PCR |
Authors: | WANG, XUEZHENG XU, BINGYIN ZHAO, LIANG GAO, PENG MA, HONGYAN LUAN, FEISHI |
Issue Date: | 13-May-2014 |
Publisher: | Karachi: Pakistan Botanical Society |
Citation: | Wang, X., Xu, B., Zhao, L., Gao, P., Ma, H., & Luan, F. E. I. S. H. I. (2014). Expression analysis of Fusarium wilt resistance gene in melon by real-time quantitative PCR. Pak J Bot, 46(2), 713-717. |
Abstract: | Melon Actin gene was used as a reference gene, to explore the gene expression profiles of the Fom-2 gene in roots, stems, and leaves of melon MR-1 under induction by Fusarium oxysporum f. sp. melonis. Monitoring using real-time quantitative PCR showed similar accumulation patterns of Fom-2 in roots, stems, and leaves over the observation period of 1 to 11 days; the expression level in stems was the highest. The expression of the Fom-2 gene was strengthened by the prolongation of induction time. In stems, the expression of Fom-2 was 5.737 times higher than in the control at three days; in roots, expression of Fom-2 was 5.617 times higher than in the control at five days. Similarly, the expression of Fom-2 in leaves obviously increased. It was 4.441 times higher than in the control at 5 days. The expression of Fom-2 was non-tissue specific, up-regulated under induction by Fusarium, and related to early resistance to Fusarium wilt. |
URI: | http://142.54.178.187:9060/xmlui/handle/123456789/15727 |
ISSN: | 2070-3368 |
Appears in Collections: | Issue 02 |
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