SSR AND SRAP MARKER BASED LINKAGE MAP OF VITIS AMURENSIS RUPR.

Abstract

SSR (Simple Sequence Repeat) markers and SRAP (Sequence Related Amplified Polymorphism) markers were used to construct a genetic linkage map of Vitis amurensis Rupr. The mapping population consists of 94 individuals derived from the selfing of Beibinghong, a V. amurensis cultivar. The map covers a total length of 1123cM on 21 linkage groups with an average distance between adjacent markers of 4.5cM and 10.9 markers per linkage group on average. The order of SSR markers in the map presented in this paper was mainly consistent with the previous published Vitis linkage maps except for few inversions. The efficiency of SSRs to anchor homologous linkage groups from different Vitis maps and the ability of SRAPs in saturating molecular linkage maps were observed. This map can serve as a fundamental tool for a series of further studies in V. amurensis, including resistance trait QTL (quantitative trait locus) detection and MAS (marker assisted selection).