RANDOM MUTAGENESIS FOR ENHANCED PRODUCTION OF ALPHA AMYLASE BY ASPERGILLUS ORYZAE IIB-30

Abstract

The current study deals with the isolation of novel mutant of A. oryzae for higher alpha amylase production. The alpha amylase producing A. oryzae strain IIB-30 was subjected to physical (UV) and chemical (Nitrous acid and EMS) mutagenic treatments to enhance the amylolytic potential of parental strain. After mutagenesis isolates were screened both qualitatively and quantitatively. Among these, EMS-20 exhibited highest enzyme activity (280±0.9 U/ml). This mutant showed 2.1 fold increased activity over the parental strain in terms of enzyme production. The result showed the EMS was effective mutagenic agent for stain improvement.