In vitro hepatoprotective activity of Sargassum wightii against CCl4 induced hepatic damage

Abstract

In vitro hepatoprotective activity of Sargassum wightii methanolic extract was checked using established cell culture (Hep G2) and primary rat hepatocytes. In Hep G2 cells, the extract concentrations varying from 31.25-500 µg/ml were studied using 1% CCl4 as toxicant. All the extracts showed moderate protectivity against toxicity induced by CCl4 in Hep G2 cells. All the biochemical constraints were determined and compared with that of the control. In this study, an innovator product (silymarin) was used along with the test extracts. In vitro hepatoprotective activity was evaluated by analyzing various parameters such as ALAT, ASAT, ALP, total protein, total bilirubin, TGL and albumin. The results stated that the hepatoprotective potential exhibited by the methanolic extract of Sargassum wightii (SWMH) is active and comparable with the standard.