Ethanolic seeds extract of Centratherum anthelminticum reduces oxidative stress in type 2 diabetes

Abstract

This work was accomplished to assess the in-vitro antiglycation and antioxidant activities of ethanolic seeds extract of Centratherum anthelminticum (CSEt), followed by its in-vivo examination in type 2 diabetes. Overnight fasted rabbits were divided into control and diabetic groups. Rabbits in diabetic group were fed with 35% fructose solution to develop hyperglycemia that was well-monitored by glucometer. These were divided into diabetic control (distilled water 1ml/kg), positive control group (pioglitazone 15mg/kg) and two test groups (CSEt 400 and 600mg treated). All treatments were given orally. After 14 days, rabbits were sacrificed and blood samples were used to estimate glycated haemoglobin (HbA1c) while total bilirubin (direct and indirect), uric acid, alanine aminotransferase (ALT) and creatine kinase (CK) were done in sera. In addition, antioxidant parameters viz., catalase (CAT), superoxide dismutase (SOD), reduced glutathione (GSH) and lipid peroxidation (LPO) were in liver tissues. The in-vitro studies showed good antiglycation and antioxidant potential of CSEt. Similarly, in-vivo investigation showed significant reduction in glycemia and body weights in type 2 diabetic test groups. Plus values of HbA1c, ALT, CK, uric acid and bilirubin were almost back to normal along with improvement found in efficiency of antioxidant parameters.