Effect of aerobic exercise combined with ginkgo polysaccharide on weight, blood glucose and glycosylated serum protein in diabetic rats

Abstract

Streptozotocin copied diabetic rats model were studied through aerobic exercise combined with ginkgo polysaccharide. Aimed to offering the updating experimental basis to prevent diabetic through aerobic exercise combined with ginkgo polysaccharide, the effect of aerobic exercise combined with ginkgo polysaccharide were discussed on the weight, blood glucose, glucosylated serum protein and the secretion ability of islet β cells, oral glucose tolerance test, islet cell pathological morophological changes and positive expression of Bax and Bcl-2. At the end of the experiment, the parameters changes were detected, which were body weight, weight of pancreas islet, Fasting blood glucose(FGB), glycosylated serum protein(GSP), FINS, C-peptide, glucose kinase (GK), ultramicro total atpase, ultramicro Na+ K+ - atpase, altramicro Ca2-M2+-atpase, β cell function index (HBCI), and oral glucose tolerance test (OGTT). HE dyeing technology was applied on insulin for pathological morphology observation. The positive expression changes of islet β cells Bcl-2 and Bax were detected by immunohistochemical staining and semi-quantitative method of stereology. Experimental results proved that body weight, GK, ultra micro Na+ K+ -atpase, ultra micro Ca2+M2+-atpase, C peptide were significantly increased after the intervention of ginkgo polysaccharide (P<0.05), correspondingly, insulin levels were dramatic increased (P<0.01), while blood glucose and GSP were decreased significantly (P<0.05), the ultramicro total atpase decrease, without statistically significance. After the exercise intervention, body weight, ultra micro Na+ K+ - atpase, ultra micro Ca2+M2+-atpase, C peptide significantly increased (P<0.05), insulin level apparently increased (P<0.01), blood glucose and GSP dramatically decreased (P<0.05), GK and ultra micro total atpase had an elevated trend, while without statistically significance. After the rational exercise combined with ginkgo polysaccharide intervention, GK, ultra micro total atpase, ultra micro Na+ K+ -atpase, ultra micro Ca2+M2+-atpase and insulin level significantly increased (P<0.05), body weight and c peptide significantly increased (P<0.01), blood glucose and GSP obviously decreased (P<0.01). The pathological changes of insulin in diabetic rats were improved. The increased ratio of Bcl-2/Bax enabled the pancreatic β cell developed in the direction of repair and regeneration. The combination of aerobic exercise and ginkgo polysaccharide could help to increase insulin secretion in diabetic rats, and increase insulin reserve and secretion capacity. Then it can control the weight boss of diabetic rats, along with the blood glucose. So it could lead to the development of the pancreas islets of diabetic rats in the direction of repair and regeneration.