Novel UV-spectrophotometric method for quantitative estimation of furazolidone using mixed hydrotropic agent

Abstract

A novel, eco friendly, accurate, sensitive, economic and safe spectrophotometric method was developed by application of mixed hydrotropy using 2 M sodium acetate, 8 M urea, 2 M niacinamide and 2 M sodium benzoate solution (25:25:25:25% V/V) as hydrotropic agent, for the solubalizing of poorly water-soluble Furazolidone (FZ) (solubility:- 3.64e-01 mg/mL in water). There were more than 32 times enhancements in the solubility of FZ were found in mixed hydrotropic solution as compared to solubilities in distilled water. FZ shows maximum absorbance at 360 nm where sodium acetate, urea, niacinamide, sodium benzoate and other tablets excipients did not show any absorbance above 300 nm, and thus no interference in the estimation was seen. FZ was obeyed Beer’s law in the concentration range of 10 to 50 µg/ml (r2=0.9992) in mixed hydrotropic solvent with mean recovery ranging from 97.32% to 98.9%. Proposed method is new, simple, economic, safe, rapid, accurate and reproducible and was validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values.