Study on effect of the expression of siRNA in gastric cancer bearing nude mice transplanted tumor NEDD9 gene

Abstract

The clinical study found that NEDD9 showed high expression on the invasion in gastric cancer tissues and metastasis of the tumor. Based on promoting the fundamental role (Sisen et al., 2013) to the expression level, the author further study NEDD9 siRNA, which could significantly reduce NEDD9 protein and mRNA in gastric cancer BGC823 cells, inhibition of cell proliferation, induce cell apoptosis, and decrease the invasiveness of gastric cancer cells, suggesting that NEDD9 plays an important role in the gastric cancer cell proliferation, apoptosis and invasion force. Through constructing a model transplanted gastric cancer in nude mice, the author observes the effect of NEDD9 siRNA on the growth of gastric cancer x-engrafts, and application of NEDD9 immunohistochemical SP method. The author also uses Western blot method to detect the gastric carcinoma in nude mice transplanted tumor tissues expression; applies situ hybridization, RT-PCR technology to detect the gastric cancer engraft tissues in NEDD9 mRNA. In order to further explore the relationship between NEDD9 and the development of gastric cancer, he provides a theoretical basis for the NEDD9 targeted therapy.