Influence of smoothened siRNA on human esophageal cancer cell line EC9706 proliferation and apoptosis

Abstract

Apply siRNA technology to take Smoothened (Smo) genetic locus, the possibility of Hh signaling pathway, and also inhibits esophageal squamous carcinoma cell proliferation and induction of apoptosis. Utilizing Smo siRNA transfected esophageal squamous carcinoma EC9706 cells, adopting RT-PCR and Western blot technology to detect Smo, Gli1 mRNA and protein expression in each group of cells, employing MTT and FCM to detect the influence of Smo siRNA on cell proliferation and apoptosis. Compared with each control group, after transfected Smo siRNA transfected cell for 24h, 48h and 72h, Smo siRNA and Gli1 mRNA expression was evenly reduced obviously, Smo and Gli1 protein expression level was also reduced clearly. Smo siRNA could inhabit cell proliferation, and even promote cell apoptosis. Smo gene played a role in regulating and controlling esophageal squamous carcinoma cell proliferation and apoptosis and Smo was likely to be an effective target spot for tumor gene therapy.