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Please use this identifier to cite or link to this item: http://142.54.178.187:9060/xmlui/handle/123456789/16219
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dc.contributor.authorHui, Wu-
dc.contributor.authorKuisheng, Chen-
dc.contributor.authorHongxin, Zhang-
dc.contributor.authorYanzhi, Ding-
dc.contributor.authorZhihua, Zhao-
dc.contributor.authorHu, Zhang-
dc.date.accessioned2023-01-20T05:53:24Z-
dc.date.available2023-01-20T05:53:24Z-
dc.date.issued2014-09-12-
dc.identifier.citationHui, W., Kuisheng, C., Hongxin, Z., Yanzhi, D., Zhihua, Z., & Hu, Z. (2014). Influence of smoothened siRNA on human esophageal cancer cell line EC9706 proliferation and apoptosis. Pakistan Journal of Pharmaceutical Sciences, 27(5).en_US
dc.identifier.issn1011-601X-
dc.identifier.urihttp://142.54.178.187:9060/xmlui/handle/123456789/16219-
dc.description.abstractApply siRNA technology to take Smoothened (Smo) genetic locus, the possibility of Hh signaling pathway, and also inhibits esophageal squamous carcinoma cell proliferation and induction of apoptosis. Utilizing Smo siRNA transfected esophageal squamous carcinoma EC9706 cells, adopting RT-PCR and Western blot technology to detect Smo, Gli1 mRNA and protein expression in each group of cells, employing MTT and FCM to detect the influence of Smo siRNA on cell proliferation and apoptosis. Compared with each control group, after transfected Smo siRNA transfected cell for 24h, 48h and 72h, Smo siRNA and Gli1 mRNA expression was evenly reduced obviously, Smo and Gli1 protein expression level was also reduced clearly. Smo siRNA could inhabit cell proliferation, and even promote cell apoptosis. Smo gene played a role in regulating and controlling esophageal squamous carcinoma cell proliferation and apoptosis and Smo was likely to be an effective target spot for tumor gene therapy.en_US
dc.language.isoenen_US
dc.publisherKarachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachien_US
dc.subjectesophageal canceren_US
dc.subjectSmo geneen_US
dc.subjectcell Proliferationen_US
dc.subjectcell Apoptosisen_US
dc.subjectRNA interferenceen_US
dc.titleInfluence of smoothened siRNA on human esophageal cancer cell line EC9706 proliferation and apoptosisen_US
dc.typeArticleen_US
Appears in Collections:Issue No.5 (Supplementary)

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