Prevention and treatment of osteoporosis caused by oestrogen shortage in rats

Abstract

Through castrated rats osteoporosis models, this study aims to explore whether the intraperitoneal injection of Scutellaria baicalensis extract could prevent the occurrence of osteoporosis and slow down/reverse osteoporosis or not, and further explore the influence on BMMSCs extracted by Scutellaria baicalensis extract. Scutellaria baicalensis extracts on the prevention and treatment of oestrogen shortage and osteoporosis. After the screening of appropriate concentration, in the model of castrated female rats with osteoporosis, rats in prevent group were given intraperitoneal injection of Scutellaria baicalensis extract within 1 week after the castration, and the rats in treatment group were injected Scutellaria baicalensis extract at the abdominal cavity in two months of castration. It required 2 times per week, 12 weeks in total, Micro CT scanning was performed respectively at 6th and 12th week on one side of the femur to compare the differences in bone mass and bone trabecular quantity, in order to evaluate the prevention and treatment of Scutellaria baicalensis extract in osteoporosis. After intraperitoneal injection of Scutellaria baicalensis extract, Micro CT results showed the animal bone mass and bone trabecular quantity in prevention and treatment group were increased compared to control group, while, it only reduced bone loss and restored bone mass to a certain extent. In this study, the small molecular compound with higher content of flavonoids through leaching, extraction, enrichment method from Traditional Chinese medicine radix scutellariae and could be utilized on the prevention and treatment of osteoporosis caused by rats castration.