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Please use this identifier to cite or link to this item: http://142.54.178.187:9060/xmlui/handle/123456789/16305
Title: Development and validation of a stability-indicating RP-HPLC-FLD method for determination of 5-[(4-chlorophenoxy) methyl]-1, 3, 4- oxadiazole-2-thiol; A novel drug candidate
Authors: Naureen Shehzadi
Khalid Hussain
Muhammad Islam
Nadeem Irfan Bukhari
Noman Asif
Khan, Muhammad Tanveer
Muhammad Salman
Shaista Qamar
Sajida Parveen
Fakhra Zahid
Arshad Ali Shah
Abida Bilal
Abbasi, Muhammad Athar
Sabahat Zahra Siddiqui
Aziz-Ur-Rehman
Keywords: Stability-indicating method
RP-HPLC-FLD
5-[(4-chlorophenoxy) methyl]-1, 3
4-oxadiazole-2-thiol
quality control analysis
Forced degradation studies
Issue Date: 4-Mar-2018
Publisher: Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi
Citation: Shehzadi, N., Hussain, K., Islam, M., Bukhari, N. I., Asif, N., Khan, M. T., ... & Siddiqui, S. Z. (2018). Development and validation of a stability-indicating RP-HPLC-FLD method for determination of 5-[(4-chlorophenoxy) methyl]-1, 3, 4-oxadiazole-2-thiol; A novel drug candidate. Pakistan Journal of Pharmaceutical Sciences, 31.
Abstract: The present study describes the development and validation of a simple high performance liquid chromatographic method for the determination of a novel drug candidate, 5-[(4-chlorophenoxy) methyl]-1, 3, 4- oxadiazole-2-thiol. The stability-indicating capacity of the method was evaluated by subjecting the compound’s solution to hydrolytic, oxidative, photolytic, transition metal- and thermal- stress. The chromatographic separation was achieved over a C18 column (Promosil, 5 µm, 4.60 × 250 mm), maintained at 25°C, using an isocratic mobile phase comprising a mixture of acetonitrile and acidified water of pH 2.67 (1:1, v/v), at a flow rate of 1.00 mL/min and detection using a fluorescent light detector (excitation at 250 nm and emission at 410 nm). The Beer’s law was followed over the concentration range 2.50-50.00 µg/mL. The recovery (98.56-100.19%, SD <5%), intraday accuracy and precision (97.31-100.81%, RSD <5%), inter-day accuracy and precision (97.50-100.75%, RSD <5%) and intermediate accuracy and precision (98.10-99.91%, RSD <5%) indicated that the method was reliable, repeatable, reproducible and rugged. The resolution and selectivity factors of the compound’s peak from the nearest resolving peak, particularly in case of dry heat and copper metal stress, were found to be greater than 2 and 1, respectively, which indicated specificity and selectivity. The compound was extensively decomposed in alkaline-hydrolytic, oxidative, metal- and dry heat- stress. However, the compound in acidic and neutral conditions was resistant to photolysis. The results of the present study indicate that the developed method is specific, selective, sensitive and suitable, hence, may be used for quality control, stability testing and preformulation studies.
URI: http://142.54.178.187:9060/xmlui/handle/123456789/16305
ISSN: 1011-601X
Appears in Collections:Issue No.2 (Supplementary)

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