Please use this identifier to cite or link to this item: http://localhost:80/xmlui/handle/123456789/16390
Title: In vitro/in vivo effect of Citrus limon (L. Burm. f.) juice on blood parameters, coagulation and anticoagulation factors in rabbits
Authors: Azra Riaz
Rafeeq Alam Khan
Talat Mirza
Tazeen Mustansir
Mansoor Ahmed
Keywords: Thrombin time
activated partial thrombin time
Fibrinogen concentration
mean corpuscular hemoglobin concentration
Issue Date: 24-Jul-2014
Publisher: Karachi: Faculty of Pharmacy, University of Karachi
Citation: Riaz, A., Khan, R. A., Mirza, T., Mustansir, T., & Ahmed, M. (2014). In vitro/in vivo effect of Citrus limon (L. Burm. f.) juice on blood parameters, coagulation and anticoagulation factors in rabbits. Pakistan journal of pharmaceutical sciences, 27(4).
Abstract: The genus Citrus of the family Rutaceae includes many species e.g. Citrus indica, Citrus aurantifolia and Citrus limon, among which Citrus limon L. Burm. f. has been reported to have highest antimicrobial activity. It is used as antidote against certain venom, due to its platelet inhibitory effect and also reported to have hypocholesterolemic effect. However its anticoagulant and thrombolytic effect were not been investigated, hence a prospective in-vitro/in-vivo study was designed to determine the effect of Citrus limon on blood parameters, coagulation and anticoagulation factors. Invitro tests revealed highly significant increase in thrombin time and activated partial thromboplastin time by Citrus limon, whereas fibrinogen concentration was significantly reduced in comparison to control, however prothrombin time was not affected significantly. In-vivo testing of Citrus limon was done at three different doses i.e. 0.2ml/kg, 0.4ml/kg and 0.6ml/kg in healthy rabbits. Significant changes were observed in hematological parameters such as erythrocytes, hemoglobin and mean corpuscular hemoglobin concentration. Bleeding time and thrombin time was significantly prolonged and there was increase in protein C and thrombin antithrombin complex levels. These results may be due to inactivation of thrombin because it significantly decreases fibrinogen concentration and inhibit platelet aggregation. Citrus limon showed maximal anticoagulant effect at 0.4ml/kg, which suggest that Citrus limon possesses an antithrombin component and could prevent thrombosis playing a cardio protective role.
URI: http://142.54.178.187:9060/xmlui/handle/123456789/16390
ISSN: 1011-601X
Appears in Collections:Issue No.4

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