Industrially relevant cellulase production by indigenous thermophilic Bacillus licheniformis TLW-3 strain: Isolation-molecular identification and enzyme yield optimization

Abstract

Cellulases are the third largest single industrial bio-robots. These enzymes are employed in industries like pharmaceutical, textile, food processing, paper recycling and detergent manufacturing. In order to produce broadly diversified cellulases, microbes (both bacteria and fungi) have been exploited. Different ecological niches have already been explored for the isolation of cellulolytic microbes. However, there have been no remarkable reports viz a viz to the hot oven ash (for cellulolytic bacterial flora). In this regard, a Bacillus strainTLW-3 was isolated and selected for CMCase production and optimization. The strain was identified as B. licheniformis TLW-3 through 16S rDNA sequencing that was submitted to Gen Bank with accession numberKY440432. The isolate growth and CMCase production conditions were optimized to get the maximum CMCase yield. The highest growth and maximum CMCase production by B. licheniformis TLW-3 were recorded at pH 7 and 50ºC, after the incubation period of 72 (hour) at 150rpm. Studies on the various nitrogen and carbon sources on CMCase production showed that the medium having 1% peptone, 0.5% yeast extract and 1% CMC can significantly enhance the enzymatic yield as compared to other (studied) sources. EDTA, Tween-20 and Tween-80 acted as inhibitors for the enzyme production. The present study holds the conviction that the (reported) organism could directly be applied to produce industrial thermophilic CMCase.