mmunohistochemical analysis of Hodgkin’s lymphoma in population of Sindh, Pakistan

Abstract

Objective: To confirm Hematoxylin and Eosin (H&E) diagnosed and suspicious cases of Hodgkin’s lymphoma then sub-classify & differentiate from other mimicries based on panel of CD markers. Methodology: This descriptive cross sectional study used non-probability purposive sampling and cases were collected at Department of Pathology, Liaquat University of Medical and Health Sciences (LUMHS) Jamshoroa and Diagnostic and Research Laboratory Hyderabad. Chi square test was used to compare discrete variables. Pearson’s correlation coefficients were used to test the correlation between continuous variables. Results: Out of 100 cases, 80 were of Hodgkin's lymphoma (HL), based on panel of Cluster differentiation (CD) markers expression. Among these, 70 were of Classical Hodgkin's lymphoma (CHL), based on either positivity of both CD30 and CD15 or any one of them with negativity of CD45 for H&RS cells. The remainders 10 were of Nodular lymphocyte predominant Hodgkin's lymphoma (NLPHL), showing positivity for CD45 and negativity for CD30 & CD15 for Lymphocytic & Histiocytic (L&H) cells in all cases, but also shown positivity for CD20 in 90% cases. Conclusion: The Hodgkin’s lymphoma originates from mature germinal center B-lymphocyte with phenotypically expression of CD 15 & CD 30, but in our investigation of immunochemistry showed that CD 20 positivity is not unusual. Lymphocytic and histiocytic cells of NLPHL expressed CD20 in 90% and CD45 in 100% cases This is mandatory for H&E diagnosed and suspicious cases of HL, to confirm on panel of CD markers, before giving the opinion.