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http://localhost:80/xmlui/handle/123456789/16857| Title: | ISOLATION AND YIELD OF PROTOPLASTS FROM DIFFERENT TISSUES OF TRITICUM AESTIVUM IN STERILE CULTURE |
| Authors: | KHALIDA KHATOON |
| Issue Date: | 4-Dec-1985 |
| Publisher: | Karachi: Pakistan Botanical Society |
| Citation: | Khatoon, K. (1985). Isolation and yield of protoplasts from different tissues of Triticum aestivum in sterile culture. Pakistan Journal of Botany. |
| Abstract: | Protoplasts from leaves, roots excised from young seedlings and calli induced from roots of seeds have been isolated with a digestion mixture containing 2.5% Macerozyme, 2.5% cellulase Onozuka-R40 and i% hemicellulase in 0.7% mannitol at pH 5.6 after 6 h incubation. Young leaves yielded highest amount of protoplasts and appeared to be a good source of plant material. |
| URI: | http://142.54.178.187:9060/xmlui/handle/123456789/16857 |
| ISSN: | 0556-2231 |
| Appears in Collections: | Issue No. 2 |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| archives2.php?vol=17&iss=2&yea=1985.htm | 133 B | HTML | View/Open |
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