THE ROLE OF MEDIUM COMPOSITION AND LIGHT INTENSITY ON IN VITRO ROOT FORMATION OF STRAWBERRY

Abstract

In vitro propagated strawberry shoots were subjected to in vitro rooting under different BA levels, types of media, incubation temperatures and light intensities. Root formation started within 10 days of culture under high light intensity of 13.5 W/rn-2. In vitro rooting of strawberry shoot tips occurred readily on inorganic salts of Murashige and Skoog medium. The extent of root formation was directly related to sucrose and light in-tensity. No root formation occurred in the dark, under low light and in sucrose free medium. BA upto 1 Jim did not inhibit root formation. Addition of nicotinic acid, pyridoxine-HCI, and thiamine-HCL to the media re-duced root formation but their effect was masked jointly by ascorbic acid, biotin, caD-pantothenate, folic acid and riboflavin.