DEVELOPMENT OF RAPD-BASED MOLECULAR MARKERS FOR CHROMOSOME 5A OF COMMON WHEAT

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Title:	DEVELOPMENT OF RAPD-BASED MOLECULAR MARKERS FOR CHROMOSOME 5A OF COMMON WHEAT
Authors:	Amjad Ali Khan, Imtiaz Ahmad Habib Ahmad Sajid-ul-Ghafoor
Issue Date:	20-Apr-2009
Publisher:	Karachi: Pakistan Botanical Society, University of Karachi
Citation:	Ali, A., Khan, I. A., Ahmad, H. A. B. I. B., & Ghafoor, S. U. (2009). Development of RAPD-based molecular markers for chromosome 5A of common wheat. Pak. J. Bot, 41(2), 691-694.
Abstract:	During the present study, 6 deletion lines (del 5AS-3, del5AS-7, del5AS-10, del5AL-10, del5AL-12 and del5AL-23) of chromosomes 5A of common wheat were used to identify RAPD (Randomly Amplified Polymorphic DNA) based molecular markers specific for short and/or long arm of chromosome 5A and one deletion line (5DS-1) used as a positive control. Out of the 7 RAPD primers used, OPA-07 showed useful polymorphism for long arm deletion line del5AL-23. By comparing the C-banding karyotype of the deletion lines, it is inferred that the primer (OPA-07) anneals to the distal half of the long arm of chromosome 5A.
URI:	http://142.54.178.187:9060/xmlui/handle/123456789/17478
ISSN:	0556-3321
Appears in Collections:	Issue No. 2

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