OPTIMIZATION OF THE PROTOCOLS FOR CALLUS INDUCTION, REGENERATION AND ACCLIMATIZATION OF SUGARCANE CV. THATTA-10

Abstract

A highly efficient protocol for the induction of callus and establishment of cell suspension culture was developed for Actinidia deliciosa. Germination of Actinidia seeds were carried out on full strength MS medium without any growth hormone and the leaves from In vitro grown plantlets were used for the induction of callus. Maximum amount of friable calli were obtained on full strength MS medium supplemented with 1.0 mg/L IBA (Indole-3-butyric acid) and 0.5 mg/L BAP (6-Benzyl amino purine) with the 16 hrs illumination period. Cell suspension cultures were established using MS medium with higher amount of IBA (2.0 mg/L) and BAP (0.5 mg/L), by rotating the culture flasks at 110 rpm on a gyratory shaker. Biotransformation ability of the suspension culture of Actinidia deliciosa was also determined by adding (-)-Ambrox to the cultures as a substrate. Six transforms were isolated after 15 days of incubation while two of them were found novel.