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Title: | EFFECT OF SINGLE AND DOUBLE WASHING ON THE LIVEABILITY OF BUFFALO BULL SPERMATOZOA AT 37°C |
Authors: | M. Ahmad S. Rehman A. Khan K.M. Ahmad |
Issue Date: | 3-Oct-1997 |
Publisher: | Faisalabad: Faculty of Veterinary Science University of Agriculture Faisalabad |
Citation: | Ahmad, M., Rehman, S., Khan, A., & Ahmad, K. M. (1997). Effect of single and double washing on the liveability of buffalo bull spermatozoa at 37 C. PAKISTAN VETERINARY JOURNAL, 17, 171-175. |
Abstract: | This srudy was conducted to investigate the effect of washing on motility, liveability (hours) and absolute index of liveability of buffalo bull spermatozoa at 37°C. Five pooled (1st and 2nd ejaculate) samples from each of the two buffalo bulls were collected. Each pooled ejaculate was divided into five aliquots (A, ro E). Of these, B to E were centrifuged at 3000 rpm for 15 minutes. The seminal plasma was removed from aliquots C, D and E while aliquot B was kept as such having intact seminal plasma. Aliquot D was given single washing while aliquot E was subjected to double washing with sodium citrate solution. The remaining fifth aliquot A served as control. Treated and control aliqouts were diluted at I: I 0 with extender (milk-egg yolk-glycerol) and incubated at 37uc. Statistical analysis of the data revealed that removal of seminal plasma and washing with sodium citrate solution have a beneficial effect on liveability of buffalo bull spermatozoa. However, double washing of spermatozoa yielded better results than other treatments as well as control. |
URI: | http://142.54.178.187:9060/xmlui/handle/123456789/17931 |
ISSN: | 0253-8313 |
Appears in Collections: | Issue 04 |
Files in This Item:
File | Description | Size | Format | |
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171-174.htm | 119 B | HTML | View/Open |
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