PREPARATION AND EVALUATION OF ALUM PRECIPITATED AND OIL-BASED HAEMORRHAGIC SEPTICAEMIA VACCINES

Abstract

Pasteurella multocida (Robert type-1) was grown on tryptose sucrose yeast (TSY) broth in a fermenter. A sterilised air was provided that potentiated the density of the culture (5 x 109 organisms/ml). The culture was inactivated with formalin (0.5% ). The alum precipitated and oil based haemorrhagic septicaemia vaccines (APHSV and OBHSV) were prepared and their efficacies were evaluated in rabbits. Both of these vaccines induced high level of indirect hemagglutination (IHA) antibody titer and 100 per<~nt protection to challenge on 90 days post vaccination. OBHSY induced higher level of antibodies than that of APHSV. However, both of these vaccines increased 2 a F body temperature of the vaccinated rabbits. The temperature incepted declining to normal on 6 hours post-vaccination.