ELECTROPHORETIC PATTERN OF POLYPEPTIDES OF INFECTIOUS BURSAL DISEASE VACCINE VIRUS

Abstract

Four live vaccines and five field isolates of infectious bursal disease virus (IBDV) were analyzed for polypeptide profile using sodium dodecyle sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Field viruses were purified and concentrated through sucrose gradient centrifugation. All the five field IBDV contained five polypeptides with molecular weights (MW) 27.70, 32.70, 41.60, 63.09 and 91.20 KDa. Out of four vaccinal strains of IBDV, only one vaccinal strain contained five polypeptides with almost similar MW i.e.,26.0, 32.0, 40.8, 62.0 and 90.9 KDa and one extra band (MW 20.3 KDa). One vaccine virus had three polypeptides with MW 40.8, 62.0 and 90.9 KDa and the remaining two vaccines contained two polypeptides with MW 40.8 and 90.9 KDa. Results reveal that vaccinal strains of IBDV differ from local field isolates in the sense of low protein profile or missing of some polypeptides.