DEVELOPMENT OF INDIRECT IMMUNOPEROXIDASE ASSAYS FOR THE DETECTION OF AVIAN ADENOVIRUSES IN INFECTED CELL CULTU

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Title:	DEVELOPMENT OF INDIRECT IMMUNOPEROXIDASE ASSAYS FOR THE DETECTION OF AVIAN ADENOVIRUSES IN INFECTED CELL CULTU
Authors:	M.D. Ahmad Burgess, G.W.
Issue Date:	5-Jul-1998
Publisher:	Faisalabad: Faculty of Veterinary Science University of Agriculture Faisalabad
Citation:	Ahmad, M. D., & Burgess, G. W. (1998). Development of indirect immunoperoxidase assays for the detection of avian adenoviruses in infected cell culture. Pakistan Veterinary Journal, 18, 142-145.
Abstract:	Monolayer indirect immunoperoxidase (liP) assays were developed for the improvement of routine viral isolation and titration. The instant identification of virus with increased sensitivity ( 10-100 fold) was the characteristic of liP. Polyclonal chicken antiserum reacted with all the avian adenoviruses (FA V) strains studied. Viral isolation and titration was made possible without the need of blind passages. However, the isolates were also characterized or typed by studying their reaction pattern with a panel of monoclonal antibodies (MAbs) produced to FA V strain 398A.
URI:	http://142.54.178.187:9060/xmlui/handle/123456789/17947
ISSN:	0253-8313
Appears in Collections:	Issue 03

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