EFFECT OF CRYOPRESERVATION ON THE POST THAW SURVIVEABILITY OF BUFFALO BULL SPERMATOZOA WITHOUT SEMINAL PLASMA

Abstract

Semen samples from Nili Ravi buffalo bulls were collected and divided for three treatments i.e. A (whole semen), B (spermatozoa free of seminal plasma) and C (spermatozoa resuspended in seminal plasma). Each frctction was extended in two different extenders viz. E1 (mi!k-egg yolk glycerol), E2 (lactose-fructoseegg yolk-glycerol) by the ratios of 1: 10. After equilibration of 5 hours, all samples wen� frozen at -196 ·�C. The average (Mean ± SD) post thaw motility (47. 75 ± 3. 87, 57.25 ± 4.38 and 34.50 ± 5.30 per-cent) and 5.30 ± 0.81, 8.00 ± 0.82 ami 3.05 ± 0.699 hours for liveability at 37JC while 102.62 ± 15.16, 217.25 ± 19.08 and 46.87 ± 9.09 for absolute index of liveability f<>r three treatments respectively. There was highly signiticant difference (P < 0.001) among the three treatment. Treatment B proved the best and followed by treatment A and C. Extender lactose-fructose-egg yolk-glycerol proved superior than milkeggyolk -glycerol.