RELEASE OF INTRACELLULAR ALANINE AND ASPARTATE TRANSAMINASE Fl�OM BUFFALO SEMEN DURING CENTRIFUGATION AND THEIR EFFECT ON SPERM SURVIVAL

Abstract

Ten pooled buffalo semen samples were separated into five equal aliquots i. e A to E. Aliquot A (whole semen not centrifuged), C (centrifuged but seminal plasma not removed) and E (centrifuged and seminal plasma removed) were extended at the ratio of 1: 10 with lactose-fructose-egg-yolk-glycerol extender and incubated to note the liveability (hours) and absolute index of liveability at 3T�·c. Aliquot E showed significant better liveability than A and C (P < 0.01). The poorest results were observed in that of aliquot c. Aliquot B (whole semen not centrifuged), D (whole semen centrifuged but seminal plasma not removed) and seminal plasma removed from aliquot E after centrifugation were used for the determination of alanine and aspartate transaminase (ALT & AST). The ALT levels of 30.29 ± 1.37, 34.75 ± 2.02 and 10.50 ± 0.48 lU/L where as AST concentnttion of 214.12 ± 3. 51, 257. 37 ± 3. 90 and 60. 28 ± 1.75 IU/L were found in aliquot B, D and E, respectively. It may he concluded from the results that centrifugation induces leakage of ALT and AST which have adverse effects on sperm survival. Therefore, seminal plasma must he removed after centrifugation to improve the sperm survivability and preservation.