RENAL CLEARANCE OF ENDOGENOUS CREATININE, UREA AND KANAMYCIN IN CO\VS

Abstract

Following a single intravenous dose of kanamycin 5 mg/kg body weight to 8 health cows, renal clearance of the drug and that of endogenous creatinine and urea were determined. Before drug au ministration control samples and after c.lrug ac.lministration blood anc.l urine samples were collectec.l at predeterminec.l time periods. The plasma anc.l urine samples wc:re assayec.l for enc.logenous creatinine anc.l urea by spectrophotometric methoc.ls anc.l kanamycin by microbiological assay. Mean ± SE (n = 32) valut!s for the: blooc.l anc.l urine pH mean were 7.37 ± 0.04 aml7 .91 ± 0.07, respectively. The rate of urine flow (diuresis) was 0.024 ± 0.003 ml.min·'.kg·', renal clearance of endogenous creatinine was 0.60 ± 0.08, urea 0.17 ± 0.04 m1c.l kanamycin was 0.08 ± 0.0 I ml.min·1.kg"1 boc.ly weight. A signiticant (P < 0.00 I) positive correlation (r=0.55) between diuresis anc.l ren�tl clearance of kanamycin inJicatt:J that renal handling of kanamycin in cows, besic.les glomerular filtration also involved ba�o:k diffusion.