INDIRECT HEMAGGLUTINATION TEST FOR THE DETECTION OF ANTIBODIES AGAINST BOOPHILUS MICROPLUS

Please use this identifier to cite or link to this item: http://localhost:80/xmlui/handle/123456789/18069

Title:	INDIRECT HEMAGGLUTINATION TEST FOR THE DETECTION OF ANTIBODIES AGAINST BOOPHILUS MICROPLUS
Authors:	C.S. Hayat Masood Akhtar Iftikhar Hussain Rehan Ahmad
Issue Date:	11-Jul-1999
Publisher:	Faisalabad: Faculty of Veterinary Science University of Agriculture Faisalabad
Citation:	Hayat, C. S., Akhtar, M., Hussain, I., & Ahmad, R. (1999). Indirect hemagglutination test for the detection of antibodies against Boophilus microplus. PAKISTAN VETERINARY JOURNAL, 19(3), 155-157.
Abstract:	Indirect hemagglutination (IHA) test for the detection of antibodies against Boophilus (B.) microplus was developed. Midgut, B. microplus, antigen was used to sensitize the human group "0" erythrocytes without coupling agent. The optimum temperature for the sensitization of erythrocytes was found to be 37°C. The sensitizing dose of the antigen was calculated as 2.0 mL per 0.01 mL of the packed erythrocytes, diluted in 2 mL phosphate buffered saline, at 37°C for 60 minutes. The IHA results were best seen after 40 minutes of adding the sensitized erythrocytes in the serially diluted samples. IHA was found to be simple, rapid and inexpensive test for the detection of anti-tick antibodies.
URI:	http://142.54.178.187:9060/xmlui/handle/123456789/18069
ISSN:	0253-8313
Appears in Collections:	Issue 03

Files in This Item:

File	Description	Size	Format
155-157.htm		119 B	HTML	View/Open

DSpace JSPUI