Please use this identifier to cite or link to this item:
http://142.54.178.187:9060/xmlui/handle/123456789/18069| Title: | INDIRECT HEMAGGLUTINATION TEST FOR THE DETECTION OF ANTIBODIES AGAINST BOOPHILUS MICROPLUS |
| Authors: | C.S. Hayat Masood Akhtar Iftikhar Hussain Rehan Ahmad |
| Issue Date: | 11-Jul-1999 |
| Publisher: | Faisalabad: Faculty of Veterinary Science University of Agriculture Faisalabad |
| Citation: | Hayat, C. S., Akhtar, M., Hussain, I., & Ahmad, R. (1999). Indirect hemagglutination test for the detection of antibodies against Boophilus microplus. PAKISTAN VETERINARY JOURNAL, 19(3), 155-157. |
| Abstract: | Indirect hemagglutination (IHA) test for the detection of antibodies against Boophilus (B.) microplus was developed. Midgut, B. microplus, antigen was used to sensitize the human group "0" erythrocytes without coupling agent. The optimum temperature for the sensitization of erythrocytes was found to be 37°C. The sensitizing dose of the antigen was calculated as 2.0 mL per 0.01 mL of the packed erythrocytes, diluted in 2 mL phosphate buffered saline, at 37°C for 60 minutes. The IHA results were best seen after 40 minutes of adding the sensitized erythrocytes in the serially diluted samples. IHA was found to be simple, rapid and inexpensive test for the detection of anti-tick antibodies. |
| URI: | http://142.54.178.187:9060/xmlui/handle/123456789/18069 |
| ISSN: | 0253-8313 |
| Appears in Collections: | Issue 03 |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 155-157.htm | 119 B | HTML | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.