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http://142.54.178.187:9060/xmlui/handle/123456789/18975| Title: | IMPROVEMENT OF SACCHAROMYCES CEREVISIAE GCU-36 THROUGH INDUCED MUTAGENESIS FOR L-PHENYLACETYLCARBINOL PRODUCTION |
| Authors: | I. Haq M. Hussain S. Ali M. M Javed M.A Qadeer |
| Keywords: | Saccharomyces cerevisiae random mutagenesis shake flask culture benzaldehyde gas chromatography L-phenylacetylcarbinol |
| Issue Date: | 2-Mar-2010 |
| Publisher: | Lahore: Pakistan Association for the Advancement of Science |
| Citation: | Haq, I. (2010). Improvement of Saccharomyces cerevisiae gcu-36 through induced mutagenesis for l-phenylacetylcarbinol production. Pakistan Journal of Science, 62(1). |
| Abstract: | The present study deals with the improvement of a Saccharomyces cerevisiae strain GCU-36 for L-phenylacetylcarbinol (L-PAC) production through randomely induced mutagenesis. The wild culture gave 2.58 g/l L-PAC production. An enhancement in the production was, however observed when yeast cells were exposed to mutagenic agents viz. ultraviolet (UV) radiations, nitrous acid (HNO2 ) and ethylmethane ulphonate (EMS). The mutant culture gave a maximal of 5.61 g/l L-PAC production which was over 2.17 fold improvement in terms of product formation. The outcome of an inferior mutant with declined L-PAC production level marked the termination of further mutagenesis. |
| URI: | http://142.54.178.187:9060/xmlui/handle/123456789/18975 |
| ISSN: | 0030-9877 |
| Appears in Collections: | Issue 01 |
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| File | Description | Size | Format | |
|---|---|---|---|---|
| PJS-255-5044.htm | 120 B | HTML | View/Open |
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