Please use this identifier to cite or link to this item: http://localhost:80/xmlui/handle/123456789/18975
Title: IMPROVEMENT OF SACCHAROMYCES CEREVISIAE GCU-36 THROUGH INDUCED MUTAGENESIS FOR L-PHENYLACETYLCARBINOL PRODUCTION
Authors: I. Haq
M. Hussain
S. Ali
M. M Javed
M.A Qadeer
Keywords: Saccharomyces cerevisiae
random mutagenesis
shake flask culture
benzaldehyde
gas chromatography
L-phenylacetylcarbinol
Issue Date: 2-Mar-2010
Publisher: Lahore: Pakistan Association for the Advancement of Science
Citation: Haq, I. (2010). Improvement of Saccharomyces cerevisiae gcu-36 through induced mutagenesis for l-phenylacetylcarbinol production. Pakistan Journal of Science, 62(1).
Abstract: The present study deals with the improvement of a Saccharomyces cerevisiae strain GCU-36 for L-phenylacetylcarbinol (L-PAC) production through randomely induced mutagenesis. The wild culture gave 2.58 g/l L-PAC production. An enhancement in the production was, however observed when yeast cells were exposed to mutagenic agents viz. ultraviolet (UV) radiations, nitrous acid (HNO2 ) and ethylmethane ulphonate (EMS). The mutant culture gave a maximal of 5.61 g/l L-PAC production which was over 2.17 fold improvement in terms of product formation. The outcome of an inferior mutant with declined L-PAC production level marked the termination of further mutagenesis.
URI: http://142.54.178.187:9060/xmlui/handle/123456789/18975
ISSN: 0030-9877
Appears in Collections:Issue 01

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