EFFECTS OF DIFFERENT PARAMETERS ON THE LIPOLYTIC ACTIVITIES OF LIPASE AND PHOSPHOLIPASE EXTRACTED FROM PRUNUS ARMINICA MEAL OF HUNZA CULTIVAR

Abstract

: Prunus Arminica.L seeds of Hunza cultivar were defatted with chloroform, methanol mixture (2:1v/v) by ultrasonic assisted extraction technique. The lipase and phospholipase extracted, incubated at 400C and pH 6 in citrate buffer for 1 hour. The lipolytic activities of enzymes were measured at different temperatures i,e 30-700C , pH i,e 3-9 and solvents i,e n-heptane , Di-isopropyl ether and Cyclo hexane. 10 % emulsion of lecithin and olive oil used as the substrate for phospholipase and lipase substrates respectively. The enzymes showed stability at lower temperatures i,e 35-500C and slightly acidic to neutral i.e pH 5 to pH 7. Concentration of liberated fatty acids was the indicator of the enzymes activity which were measured by taking absorbance at 440nm. Stearic acid with different concentrations in hexane and methanol (1:1v/v) were used for the standard curve. Cu-TEA reagent with small quantity of CaCl2 was used for maximum extraction of Cu-FFA and for stable value of blanks in the present study. The enzymes showed maximum activities at 450 C temperature and 6 pH