Please use this identifier to cite or link to this item: http://localhost:80/xmlui/handle/123456789/19111
Title: BIOFILM FORMATION AND CALCIUM PRECIPITATION IN BACILLUS LICHENIFORMIS AND ALCALIGENES FAECALIS FOR SOIL IMPROVEMENT
Authors: B. Zeb
B. Zainab
J. Siddique
K. Zahid
N. Khaliq
A.W. Qurashi
Keywords: Calcium precipitation
Bacillus licheniformis
Alcaligenes faecalis
biofilm formation and salt stress.
Issue Date: 20-Jun-2017
Publisher: Pakistan Association for the Advancement of Science,Pakistan
Citation: Zeb, B. (2017). BIOFILM FORMATION AND CALCIUM PRECIPITATION IN BACILLUS LICHENIFORMIS AND ALCALIGENES FAECALIS FOR SOIL IMPROVEMEN. Pakistan Journal of Science, 69(2).
Abstract: Calcium precipitation and biofilm formation in halophilic bacteria were investigated in the current study. The strain SMK (Bacillus licheniformis) was isolated from plant rhizosphere and and AQ-1 (Alcaligenes faecalis) from marble crushing soil in Lahore. Bacterial growth, biofilm formation and CaCO3 precipitation of both strains was determined under varying stress (pH, temperature, agitation and salinity). Soil aggregation was investigated under salt stress (NaCl and MgSO4). Results showed that pH 8.0, temperature 42°C, 1.5 M salt (NaCl and MgSO4) stress without agitation was favorable for biofilm formation. Both strains were able to precipitate CaCO3 in media specially at margins of colonies in AQ-1. Effervescence of crystals with addition of concentrated HCl showed confirmatory results. Stereomicroscopic analysis of aggregates in inoculated soil and sand showed precipitation under salt stress. SMK (Bacillus licheniformis) showed high aggregation in soil. From the results, it was concluded that these indigenous strains have potential for sustainable agriculture by improving disturbed soil.
URI: http://142.54.178.187:9060/xmlui/handle/123456789/19111
ISSN: 0030-9877
Appears in Collections:Issue 02

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