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  3. Pakistan Science Foundation
  4. Pakistan Science Foundation
  5. PSF Research Support (Scientific Research Journals)
  6. Journal of the Chemical Society of Pakistan
  7. Volume 31
  8. Issue 05
Please use this identifier to cite or link to this item: http://142.54.178.187:9060/xmlui/handle/123456789/19632
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dc.contributor.authorKHAN, HASAN MAHMOOD-
dc.contributor.authorKHAN, ASHFAQ AHMAD-
dc.date.accessioned2023-09-18T07:23:09Z-
dc.date.available2023-09-18T07:23:09Z-
dc.date.issued2009-10-20-
dc.identifier.citationKhan, H. M., & Khan, A. A. (2009). Indentification of radiation treatment of frozen chicken and fresh turkey using DNA comet assay. Journal of the Chemical Society of Pakistan, 35(5), 726-730.en_US
dc.identifier.issn0253-5106-
dc.identifier.urihttp://142.54.178.187:9060/xmlui/handle/123456789/19632-
dc.description.abstractDNA comet assay was applied to check the detection of radiation treatment of frozen chicken and fresh turkey. The cells from unirradiated and irradiated samples of chicken and turkey were extracted in cold PBS, embedded in agarose on microscope slides, lysed for 15 minutes in 2.5 % SDS and subjected to electrophoresis at a rate of2V/cm for 2 minutes. After silver staining these slides were evaluated through an ordinary transmission microscope. In irradiated samples, fragmented DNA (due to radiation treatment) stretched towards the anode and cells appear as comets. The density of DNA material in the tails increased with increasing radiation dose. However in case of unirradiated samples, the large molecule of DNA remained relatively intact and there was only minor or no migration of DNA; thus cells were round or had very short tails. Therefore, clear discrimination between unirradiated and irradiated food samples is possible. Hence, DNA comet assay provides an inexpensive and quick screening method for several kinds of foods containing DNA including frozen chicken and fresh turkeyen_US
dc.description.sponsorshipThe chemical society of Pakistan is an approved society from the PSF.en_US
dc.language.isoenen_US
dc.publisherHEJ Research Institute of Chemistry, University of Karachi, Karachi.en_US
dc.titleIdentification of Radiation Treatment of Frozen Chicken and Fresh Turkey using DNA Comet Assayen_US
dc.typeArticleen_US
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