Capillary Gas Chromatographic Determination of Dopamine by Pre-column Derivatization Method using Trifluoroacetylacetone as Derivatizing Reagent

Abstract

Capillary gas chromatographic separation of putrescine (Pu), cadaverine (Cd), dopamine (DA) and octopamine (OA) as derivatives of trifluoroacetylacetone (FAA) has been carried out using a column HP-5 (30 m × 0.32 mm id), temperature 100 ºC for 1 min, followed by heating rate 10 ºC/min up to 250 ºC using FID detection. Nitrogen flow rate was 4 mL/min and split ratio was 10:1 v/v. The linear calibration graphs were ranging between 2.5-25 μg/mL. The method was applied for the determination dopamine in pharmaceutical preparations with relative standard deviation (RSD) within 1.5-2.5% and recovery of 98% with RSD 1%.