Antiproliferative Activity and Chemical Constituents of Hypericum oblongifolium

Abstract

The antiproliferative activity of hexane (F1), ethyl acetate (F2), butanol (F3) and water (F4) extracts of H. oblongifolium were tested in vitro for their anti- proliferative (anticancer) activity on the cell lines: HT-29 human colon adenocarcinoma, NCI-H460 human non-small cell lung carcinoma, MCF-7 human breast cancer, OVCAR-3 human ovarian adenocarcinoma and RXF-393 human renal cell carcinoma with etoposide as positive control. Among the various extracts the F1 showed relatively potent anti-proliferative activity (10.55 ± 4.19 µg/mL) on OVCAR-3 human ovarian adenocarcinoma cell growth almost equal to the activity of etoposide (9.42 ± 1.62 µg/mL). Seven compounds were also isolated for the first time from this source. These phytochemicals were identified as 18βH-urs-20 (30)-en-3β-ol-28-oic acid (1), tetracosyl 3-(3,4-dihydroxyphenyl) acrylate (2), Shikimic acid (3), 1-Octatriacontanol (4), Hexacosyl tetracosanoate (5), β-Sitosterol (6) and β-Sitosterol3-O-β-D-glucopyranoside (7) on the basis of spectroscopic studies such as 1H NMR ,13C NMR, 2D NMR and Mass spectroscopy as well as established with help of reported literature.