Please use this identifier to cite or link to this item: http://localhost:80/xmlui/handle/123456789/19852
Title: Determination of Synthetic Phenolic Antioxidants in Cake by HPLC/DAD after Mixed Micelle–Mediated Cloud Point Extraction
Authors: Peijin Wang
Dongling Meng
Chang liu
Yaling Yang
Keywords: Cloud-point extraction
Mixed micelle
, Cake, High-performance liquid chromatography
Synthetic phenolic antioxidants
Issue Date: 20-Oct-2013
Publisher: HEJ Research Institute of Chemistry, University of Karachi, Karachi.
Citation: Wang, P., Meng, D., Liu, C., & Yang, Y. (2013). Determination of Synthetic Phenolic Antioxidants in Cake by HPLC/DAD after Mixed Micellea" Mediated Cloud Point Extraction. Journal of the Chemical Society of Pakistan, 35(5).
Abstract: A mixed micelle-mediated cloud point extraction (MMCPE) system was developed for the extraction and preconcentration of four synthetic phenolic antioxidants (SPAs) (propyl gallate (PG), tert-butylhydroquinone (TBHQ), butylated hydroxyanisole (BHA) and octyl gallate (OG) ) in cake. The mixture of two kinds of non-ionic surfactants polyoxy ethrlene nonyl phinyl ether (NP-7) and polyoxy ethrlene nonyl phinyl ether (NP-9) was utilized as a suitable micellar medium for preconcentration and extraction of SPAs. The surfactant-rich phase was then analyzed by high performance liquid chromatography-diode array detection (HPLC–DAD). The effect of different parameters such as concentration of surfactants, proportion of NP-7 and NP-9, equilibration time and temperature on the cloud point extraction (CPE) was carefully optimized. Under the studied conditions, four SPAs were successfully separated within 12 min. The relative standard deviations (RSD, n=6) were 1.2–2.0% and the limits of detection (LOD) were 1.5 ng mL₋1 for PG, 3.6 ng mL-1 for TBHQ, 2.9 ng mL-1 for BHA, and 0.8 ng mL-1 for OG, respectively. Recoveries of the SPAs in spiked cake samples were in the range of 92% to 99%. The MMCPE method showed potential advantage for the preconcentration of the SPAs, with enrichment factor of 25. Moreover, the method is simple, has high sensitivity, consumes much less solvent, and has significant advantage in extraction efficiency compared to traditional CPE methods.
URI: http://142.54.178.187:9060/xmlui/handle/123456789/19852
ISSN: 0253-5106
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