Gas Chromatographic Mass Spectrometric Analysis of n-Hexane Fraction of the Leaves of Sugar Beets (Beta vulgarus)

Abstract

The n-hexane fraction of the leaves of sugar beet was analyzed qualitatively and quantitatively using GC-MS and GC equipped with FID in separate experiments. The crude was extracted using ethanol and fractionated by n-hexane, ethyl acetate and chloroform in the increasing order of polarity. Only the n-hexane fraction was analyzed using GC-MS and GC equipped with FID. The nature of products was confirmed using total ion chromatograms as well as fragmentation pattern. The concentration of the major compounds was determined using the peak area. The n-hexane fraction was found to contain esters in addition to small quantities of other compounds.