Reversal of Paracetamol Induced Hepatotoxicity in Animals Model: Clove, an Alternative Medicine beyond the Spices

Abstract

INTRODUCTION: Clove is used as spice commonly and exerts beneficial effects in different human pathologies especially diabetes and infectious diseases discovered in recent times. OBJECTIVE: Current study was aimed to investigate the effects of clove extract on paracetamol induced liver toxicity. METHODOLOGY: This animal study was conducted in Department of pharmacology, Isra University Laboratory Hyderabad and at animal house of veterinary department, Sindh Agriculture University, Tando Jam between September 2013 to October 2013. Through non probability purposive sampling, 30 Rabbits were divided into three groups I, II and III. Acetaminophen 500mg was given to group II and III for 10 days orally, while group I served as control. Group III was co administered 100mg of clove powder for 20 days while group II was started 100mg clove powder on 11th day till 20th day of experiment. All blood samples were taken from ear lobe Base line blood sample before intervention was taken at day 0, then 2nd and 3rd samples were taken at 10 and 20 days respectively after intervention. Liver enzymes were also measured. Data was analyzed on SPSS version 21 using t-test, keeping P-value < 0.05 significant. RESULTS: No significant rise in liver enzymes noticed in group I and group III while all enzymes were increased in group II in initial 10 days but declined back in next 10 days. P-value calculated was 002, 0.001 and 0.001 for ALT,AST and GGT respectively. CONCLUSION: Paracetamol induced elevation of hepatic enzymes was reversed by clove.