PRESSURIZED HOT WATER EXTRACTION OF CARRAGEENAN AND PHENOLIC COMPOUNDS FROM EUCHEUMA COTTONII AND GRACILARIA SP.: EFFECT OF EXTRACTION CONDITIONS

Abstract

This work was focused on the investigation of the extraction parameters influence on the yield of carrageenan and phenolic components as well as the antioxidant activity of extracts. Pressurized hot water extraction of carrageenan and phenolic components from Eucheuma cottonii (E. cottonii) and Gracilaria sp. was performed in a semi-batch system at various pressures of 1 - 10 MPa and temperatures of 120 - 200 o C. Carrageenan and phenolic compounds were characterized by using FT-IR and UV-vis spectrophotometer. The yields of carrageenan and phenolic components were strongly influenced by the alteration of extraction temperature. The highest extraction yield was resulted at 200 o C for 97% of carrageenan yield and 22 mg gallic acid equivalent (GAE)/g of dried sample for phenolic components extracted from E. cottonii. The results confirmed that pressurized hot water extraction is an effective technique to isolate bioactive components from macroalgae and can be an advanced technique for utilization of biomass components.