PHYTOCHEMICAL ANALYSIS AND ANTIFUNGAL POTENTIAL EVALUATION OF Phyllanthus emblica

Abstract

The Present research work was conducted to examine phytochemical analysis and antifungal activity of Phyllanthus emblica. Extraction was performed through maceration method using different solvents and analyzed for the occurrence of secondary metabolites. Positive results were obtained for Saponins, Tannins, Falvonoids and Cardiac glycosides whereas negative results observed for Alkaloids, Anthraquinones and Phlobotanins. Antioxidant activity of plant extracts was also checked which showed strong results for Antioxidant activity as compared to commercially available standard antioxidants. In total antioxidant assay methanol extract of plant possesses high antioxidant potential showing the value 0.44 ± 0.134a which was closer to the standard BHT (Standard) 0.479 ± 0.24a . In case of DPPH assay chloroform extract value (0.213 ± 0.041) was found closer to BHT (Standard) when compared, whose value is 0.190 Antifungal activity of plant was carried out against fungal strains (Botrytis cinerea and Aspergilus niger), zones of inhibition were measured and compared with the commercially available standard antifungal discs (Itraconazole and Fluconazole). The highest value against B. cinerea was observed for chloroform extracts 20.83 nm, whereas in case of A. niger distilled water showed maximum value of 16.5 nm. Based on the results obtained in the present study, Phyllanthus emblica fruits are rich in phytochemical constituents and possess potent antifungal activity against pathogenic fungi and can be used in treating various diseases caused by these fungal species.