SEPARATION OF PHENOLIC GLYCOLIPIDS IN MYCOBACTERIUM BOVIS BCG BY REVERSED-PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

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Title:	SEPARATION OF PHENOLIC GLYCOLIPIDS IN MYCOBACTERIUM BOVIS BCG BY REVERSED-PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
Authors:	SALIMI, MONA POURABDI, LADAN ZAKERI, MARYAM ABDIPOUR, NAZANIN
Keywords:	HPLC-UV mycobacterium phenolic glycolipid
Issue Date:	5-Jul-2009
Publisher:	Karachi: Faculty of Pharmacy & Pharmaceutical Sciences, Karachi
Citation:	Salimi, M., Pourabdi, L., Zakeri, M., & Abdipour, N. (2009). Separation of phenolic glycolipids in mycobacterium bovis BCG by reversed-phase high performance liquid chromatography. Pakistan Journal of Pharmaceutical Sciences, 22(2).
Abstract:	A crude phenolic glycolipid extract from Mycobacterium bovis BCG was fractionated by column chromatography. A reversed-phase high performance liquid chromatography (HPLC) method with UV detection at 275nm was developed for simultaneous detection and separation of phenolic glycolipids (PGLs) in Mycobacterium bovis BCG. This analysis provides a good resolution. Different solvent systems and columns for HPLC were compared. A system composed of acetonitrile-water in the ratio of 0→80% at a flow rate of 0.8 mL/min and C8 analytical column were found to be optimum for HPLC of the phenolic glycolipids. This simple method is therefore appropriate to purify these compounds present in M. bovis extract.
URI:	http://142.54.178.187:9060/xmlui/handle/123456789/14123
ISSN:	1011-601X
Appears in Collections:	Issue 02

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