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Title: | Polymorphism of Interleukin-10 (IL-10, -1082 G/A) and Interleukin28B (IL-28B, C/T) in pediatric Acute Lymphoblastic Leukemia (ALL) |
Authors: | Ghufran, Hafiz Riaz, Sehar Mahmood, Nasir Yaqoob, Muhammad Shahid, Saman Bhinder, Munir Ahmad Anwar, Saadia Faizan, Mahwish |
Keywords: | Acute lymphoblastic leukemia (ALL) Interleukin 10 (IL-10) Interleukin 28B (IL-28B) Single nucleotide polymorphism (SNP) |
Issue Date: | 21-Sep-2019 |
Publisher: | Karachi: Faculty of Pharmacy & Pharmaceutical Sciences, Karachi |
Citation: | Syedbasha, M. (2019). The immune modulatory role of interferon lambda on human B-cell functions (Doctoral dissertation, University_of_Basel). |
Abstract: | We conducted genotypic analyses of interleukin-10 (IL-10) (-1082 G/A; GG, GA, AA) and interleukin-28B (CC, CT, TT) genes polymorphisms in acute lymphoblastic leukemia (ALL) pediatric patients in descriptive study to evaluate the prevalence of these mutations. In amplification refractory mutation system-PCR (ARMS-PCR), one reaction was carried out for each patient’s DNA sample. For IL-28B gene, two forward and two reverse primers specific for Callele and T-allele were used separately. For human IL-10 gene, two different forward primers specific for A and G alleles were used in combination with common reverse primer. IL-10 gene promoter showed highest frequency (n=29, 58%) of heterozygous (GA) allele, while genotypic analysis of IL-28B gene showed highest frequency (n=28, 56%) of homozygous (CC) allele. The IL-10 (AA) genotype related to its protein’s less production in body which may be associated with the least survival of ALL’s patients, while IL-28b (CT and TT) genotypes may be associated with less IFNλ3 levels and less life expectancy. |
URI: | http://142.54.178.187:9060/xmlui/handle/123456789/14668 |
ISSN: | 1011-601X |
Appears in Collections: | Issue 5 (Supplementary) |
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