Please use this identifier to cite or link to this item: http://localhost:80/xmlui/handle/123456789/14668
Title: Polymorphism of Interleukin-10 (IL-10, -1082 G/A) and Interleukin28B (IL-28B, C/T) in pediatric Acute Lymphoblastic Leukemia (ALL)
Authors: Ghufran, Hafiz
Riaz, Sehar
Mahmood, Nasir
Yaqoob, Muhammad
Shahid, Saman
Bhinder, Munir Ahmad
Anwar, Saadia
Faizan, Mahwish
Keywords: Acute lymphoblastic leukemia (ALL)
Interleukin 10 (IL-10)
Interleukin 28B (IL-28B)
Single nucleotide polymorphism (SNP)
Issue Date: 21-Sep-2019
Publisher: Karachi: Faculty of Pharmacy & Pharmaceutical Sciences, Karachi
Citation: Syedbasha, M. (2019). The immune modulatory role of interferon lambda on human B-cell functions (Doctoral dissertation, University_of_Basel).
Abstract: We conducted genotypic analyses of interleukin-10 (IL-10) (-1082 G/A; GG, GA, AA) and interleukin-28B (CC, CT, TT) genes polymorphisms in acute lymphoblastic leukemia (ALL) pediatric patients in descriptive study to evaluate the prevalence of these mutations. In amplification refractory mutation system-PCR (ARMS-PCR), one reaction was carried out for each patient’s DNA sample. For IL-28B gene, two forward and two reverse primers specific for Callele and T-allele were used separately. For human IL-10 gene, two different forward primers specific for A and G alleles were used in combination with common reverse primer. IL-10 gene promoter showed highest frequency (n=29, 58%) of heterozygous (GA) allele, while genotypic analysis of IL-28B gene showed highest frequency (n=28, 56%) of homozygous (CC) allele. The IL-10 (AA) genotype related to its protein’s less production in body which may be associated with the least survival of ALL’s patients, while IL-28b (CT and TT) genotypes may be associated with less IFNλ3 levels and less life expectancy.
URI: http://142.54.178.187:9060/xmlui/handle/123456789/14668
ISSN: 1011-601X
Appears in Collections:Issue 5 (Supplementary)

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