DSpace logo

  1. Pastic Digital Repository
  2. MoST Organizations
  3. Pakistan Science Foundation
  4. Pakistan Science Foundation
  5. PSF Research Support (Scientific Research Journals)
  6. Pakistan Journal of Botany
  7. Volume
  8. Volume 45
  9. Issues
  10. Issue 5
Please use this identifier to cite or link to this item: http://142.54.178.187:9060/xmlui/handle/123456789/15827
Full metadata record
DC FieldValueLanguage
dc.contributor.authorLI, JIE-
dc.contributor.authorKUANG, PING-
dc.contributor.authorLIU, REN-DAO-
dc.contributor.authorWANG, DAN-
dc.contributor.authorWANG, ZHI-NA-
dc.contributor.authorHUANG, MIN-REN-
dc.date.accessioned2023-01-10T04:12:33Z-
dc.date.available2023-01-10T04:12:33Z-
dc.date.issued2013-11-13-
dc.identifier.citationLi, J., Kuang, P., Liu, R. D., Wang, D., Wang, Z. N., & Huang, M. R. (2013). Transfer of the gafp and npi, two disease-resistant genes, into a phalaenopsis by Agrobacterium tumefaciens. Pakistan Journal of Botany, 45(5), 1761-1766.en_US
dc.identifier.issn2070-3368-
dc.identifier.urihttp://142.54.178.187:9060/xmlui/handle/123456789/15827-
dc.description.abstractGastrodia Antifungal Protein (GAFP) and Neutrophils Peptide-I (NPI), two disease-resistant genes were successfully transferred into the Dtps.Tailin Angel Phalaenopsis by using Agrobacterium strain off gradual methods. Two hundred and eighty kanamycin-resistant plants were regenerated by this method. PCR, Southern blot and RT-PCR confirmed that GAFP and NPI genes have been integrated into the genome of Phalaenopsis. In vitro antibiosis assay of the Colletotrichum gloeosporioides Sacc suggested that the transgenic plants were disease-resistant. Disease-resistance experiments proved that both GAFP and NPI genes were expressed efficiently in Phalaenopsis.en_US
dc.language.isoenen_US
dc.publisherKarachi: Pakistan Botanical Societyen_US
dc.titleTRANSFER OF THE GAFP AND NPI, TWO DISEASE-RESISTANT GENES, INTO A PHALAENOPSIS BY AGROBACTERIUM TUMEFACIENSen_US
dc.typeArticleen_US
Appears in Collections:Issue 5

Files in This Item:
File Description SizeFormat 
40.htm115 BHTMLView/Open
Show simple item record


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.