TRANSFER OF THE GAFP AND NPI, TWO DISEASE-RESISTANT GENES, INTO A PHALAENOPSIS BY AGROBACTERIUM TUMEFACIENS

Abstract

Gastrodia Antifungal Protein (GAFP) and Neutrophils Peptide-I (NPI), two disease-resistant genes were successfully transferred into the Dtps.Tailin Angel Phalaenopsis by using Agrobacterium strain off gradual methods. Two hundred and eighty kanamycin-resistant plants were regenerated by this method. PCR, Southern blot and RT-PCR confirmed that GAFP and NPI genes have been integrated into the genome of Phalaenopsis. In vitro antibiosis assay of the Colletotrichum gloeosporioides Sacc suggested that the transgenic plants were disease-resistant. Disease-resistance experiments proved that both GAFP and NPI genes were expressed efficiently in Phalaenopsis.