FUNCTION ANALYSIS OF PHYTOCHROME B GENE IN MAIZE

Abstract

ZmPHYB1 and ZmPHYB2 with some other factors and their own interactions were studied using yeast two-hybrid technology. Results showed that interaction signals were detected among different segments. Results speculated that interaction signals between ZmPHYB1 and ZmPHYB2 could be detected under certain lighting condition after a certain period of time. AtSPA1 and AtCOP1 may form complexes with other interaction factors co-interacting ZmPHYB accumulation. Agrobacterium-mediated genetic transformation study of functional ZmPhyB1 and ZmPhyB2 into Arabidopsis thaliana showed that genes can complement the corresponding mutant phenotype, while over-expressing transgenic plants gave rise to more obvious phenotypes in photo-morphogenesis. We speculated that endogenous PhyB gene interferences were responsible for different of indexes of necessary light-inducing phenotypic conditions between over-expression transform-ants and complementary transform-ants.