TOTAL PHENOLICS, PHENYL ALANINE AMMONIA LYASE AND POLYPHENOL OXIDASE IN IN VITRO CALLI OF CHICKPEA

Abstract

Conditions for in vitro cultures of calli from root, cotyledon and hypocotyl explants of chickpea (Cicer arietinum L. cv. CM 72) in M.S. medium were established. Total phenolics, phenylalanine ammonia-lyase (PAL) and polyphenol oxidases (PPO) were estimated quantitatively from main callus to 6th sub-culture. The activity of PAL and PPO showed a linear increase upto third sub-culture and then a remarkable decrease from fourth to sixth sub-culture was observed. rIhe data of phenolics was not in conformity with PAL and PPO activity. A gradual increase in total phenolics from main to sach subculture was observed. The data obtained from PAL, PP() and total phenolics correlates with the callus growth.