TOTAL PHENOLICS, PHENYL ALANINE AMMONIA LYASE AND POLYPHENOL OXIDASE IN IN VITRO CALLI OF CHICKPEA

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Title:	TOTAL PHENOLICS, PHENYL ALANINE AMMONIA LYASE AND POLYPHENOL OXIDASE IN IN VITRO CALLI OF CHICKPEA
Authors:	JAVED IQBAL SHAGUFTA NAZ SARWAT NAZIR M.S. AHMED FAHEEM AFTAB
Issue Date:	20-Dec-1991
Publisher:	Karachi: Pakistan Botanical Society, University of Karachi
Citation:	Iqbal, J., Naz, S., Nazir, S., Aftab, F., & Ahmed, M. S. (1991). TOTAL PHENOLICS, PHENYL ALANINE AMMONIA-LYASE AND POLYPHENOL OXIDASE IN INVITRO CALLI OF CHICKPEA. Pakistan Journal of Botany, 23(2), 227-235.
Abstract:	Conditions for in vitro cultures of calli from root, cotyledon and hypocotyl explants of chickpea (Cicer arietinum L. cv. CM 72) in M.S. medium were established. Total phenolics, phenylalanine ammonia-lyase (PAL) and polyphenol oxidases (PPO) were estimated quantitatively from main callus to 6th sub-culture. The activity of PAL and PPO showed a linear increase upto third sub-culture and then a remarkable decrease from fourth to sixth sub-culture was observed. rIhe data of phenolics was not in conformity with PAL and PPO activity. A gradual increase in total phenolics from main to sach subculture was observed. The data obtained from PAL, PP() and total phenolics correlates with the callus growth.
URI:	http://142.54.178.187:9060/xmlui/handle/123456789/16723
ISSN:	0556-3321
Appears in Collections:	Issue No. 2

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